To form a smooth gradient and a preparatory liquid chromatographic system
having a plurality of syringe type pumps, solvents are alternately drawn
and inserted into each cylinder. The solvents are alternately drawn into
and through a long narrow path formed of tubing to create axial mixing
and spreading out of the interface and then are drawn into the cylinders
during the refill stroke of the piston at a sufficiently high velocity to
create turbulence. The drawing of the solvents into one side of the
cylinder a radial distance from the center creates circular eddy
currents.