A queen bee rearing and introduction system which has a base portion that includes a queen bee emerging hole disposed therein and an adjacent cell cup insertion hole disposed on a first side. On an opposite, second side there is a frame retention tab for attaching the queen bee rearing system into a standard wood hive box frame disposed along its length. The base portion also has at least one cage latch. There is a cage portion that has one open end and one enclosed end. The open end has at least one cage latch aperture. When a queen cell cup containing a queen bee cell is inserted into the cell insertion hole of the base portion, the open end of the cage portion is slid over the queen cell cup and firmly snapped into place around the base portion by sliding the cage latch of the base portion into the cage latch aperture of the cage portion.