An interface for a bus test instrument is readily adaptable for testing a wide range of bus types. The interface includes a pair of transmit lines and a pair of receive lines. A transmitting circuit is adaptable for transmitting either single-ended or differential signals over the transmit lines, and at least one receiving circuit is adaptable for receiving either single-ended or differential signals from either the receive lines or the transmit lines. The flexible interface allows the testing of single-ended and differential busses, as well as busses that support both unidirectional and bidirectional communication.