Site-specific Listeria integration vectors and methods for their use are
provided. The subject vectors include a bacteriophage integrase gene and
a bacteriophage attachment site, where in many embodiments the
bacteriophage that is the source of these elements is a listeriophage. In
certain embodiments, the subject vectors further include a multiple
cloning site, where the multiple cloning site may further include a
polypeptide coding sequence, e.g., for a heterologous antigen. The
subject vectors and methods find use in a variety of different
applications, including the study of Listeria species and the preparation
of Listeria vaccines.