The invention describes a PS4 variant polypeptide derivable from a parent
polypeptide having amylase activity selected from the group consisting
of: (a) a polypeptide comprising an amino acid mutation at each of
positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272,
303, 307, 309 and 334; (b) a polypeptide comprising an amino acid
mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178,
179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an
amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157,
178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide
comprising an amino acid mutation at each of positions 3, 33, 34, 70,
121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334;
with reference to the position numbering of a Pseudomonas saccharophilia
exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as
a food or feed additive, and nucleic acids encoding such.