The biosensor comprises a modular biorecognition element and a modular
flexible arm element. The biorecognition element and the flexible arm
element are each labeled with a signaling element. The flexible arm
contains an analog of an analyte of interest that binds with the
biorecognition element, bringing the two signaling elements in close
proximity, which establishes a baseline fluorescence resonance energy
transfer (FRET). When an analyte of interest is provided to the
biosensor, the analyte will displace the analyte analog, and with it, the
signaling module of the modular flexible arm, causing a measurable change
in the FRET signal in a analyte concentration dependent manner. The
modularity of different portions of the biosensor allows functional
flexibility. The biosensor-operates without additional development
reagents, requiring only the presence of analyte or target for function.