The invention relates to a method and a device which are used to obtain and maintain multiple biological cultures, with environmental parameters similar to those of larger bioreactors (from 1 L) and with a degree of measurement automation similar to that currently obtained using microplates (1-2 mL). The aforementioned environmental parameters include at least the following: measurement and control of temperature, agitation and aeration, measurement and control of pH and dissolved oxygen and measurement of the biomass.