The present invention relates to a genetically engineered, CD19-specific
chimeric T cell receptor and to immune cells expressing the chimeric
receptor The present invention also relates to the use of such cells for
cellular immunotherapy of CD9.sup.+ malignancies and for abrogating any
untoward B cell function. The chimeric receptor is a single chain
scFvFc:.zeta. receptor where scFvFc designates the extracellular domain,
scFv designates the V.sub.H and V.sub.L chains of a single chain
monoclonal antibody to CD19, Fc represents at least part of a constant
region of an IgG.sub.1, and .zeta. represents the intracellular signaling
domain of the zeta chain of human CD3. The extracellular domain scFvFc
and the intracellular domain .zeta. are linked by a transmembrane domain
such as the transmembrane domain of CD4. In one aspect, the chimeric
receptor comprises amino acids 23-634 of SEQ I DNO:2. The present
invention further relates to a method of making a redirected T cell
expressing a chimeric T cell receptor by electroporation using naked DNA
encoding the receptor.