Provided is a method for producing a cloned dog by enucleating an oocyte
of a dog to produce an enucleated oocyte, transferring a somatic cell of
the dog into the enucleated oocyte, carrying out electrofusion under
optimized conditions to produce a nuclear transfer embryo, and
transferring the nuclear transfer embryo into its surrogate mother.
The method has the effect of producing a cloned dog with high efficiency,
and thus can contribute to the development of studies in veterinary
medicine, anthropology and medical science, such as the propagation of
superior species, xenotransplantation and diseased animal models. In
addition, the present invention has the effect of exactly researching
production properties of the cloned dog by producing a female cloned dog
for the first time, rather than the conventional male cloned dog.