An optical pickup calibrating apparatus for calibrating the laser emitting direction of an optical pickup supported on a guiding rod is provided. The optical pickup calibrating apparatus has a base and a holder. The holder, which is pivotally assembled to the base, has a trench formed on a sidewall thereof and encircling the holder. The latitude of the trench is uneven. The guiding rod has a first end slidably assembled in the trench. The rotation of the holder results a motion of the first end perpendicular to the base for adjusting the tilt of the guiding rod.