A composition having two separate phases, one above the other, the composition containing: (1) a bottom aqueous phase, (2) a top oil-in-water emulsion, and (3) at least one anionic surfactant chosen from the sodium salts of esters of dimethicone copolyol and sulphosuccinic acid. The two-phase composition has good cosmetic qualities, it is very soft and fresh upon application, while having good efficacy. The invention also relates to the use of this composition in cosmetics, especially for removing makeup from, cleansing and/or caring for the skin, the lips and/or the eyes, or for hair care.