The production of a diutan polysaccharide exhibiting increased viscosity
properties as compared with previously produced polysaccharide of the
same type of repeating units. Such an improved diutan polysaccharide is
produced through the generation of a derivative of Sphingomonas sp. ATCC
53159 that harbors a multicopy broad-host-range plasmid into which genes
for biosynthesis of diutan polysaccharide have been cloned. The plasmid
provides the capability within the host Sphingomonas strain to produce
multiple copies of genes for such polysaccharide synthesis. In such a
manner, a method of not just increased production of the target diutan
polysaccharide, but also production of a diutan polysaccharide of
improved physical properties (of the aforementioned higher viscosity)
thereof is provided. Such a diutan polysaccharide has proven particularly
useful as a possible viscosifier in oilfield applications and within
cement materials. The inventive methods of production of such an improved
diutan polysaccharide, as well as the novel cloned genes required to
produce the improved diutan within such a method, are also encompassed
within this invention. Additionally, the novel engineered Sphingomonas
strain including the needed DNA sequence is encompassed within this
invention.