In an over-sampled maximum-likelihood sequence estimation (MLSE) receiver system, the optimal sample spacing is determined for a variety of conditions. In an illustrative implementation, the system includes an optical filter for tightly filtering an incoming optical data signal with an on-off-keying (OOK) non-return-to-zero (NRZ) format, followed by an optical-to-electrical converter, an electrical filter, a sampler, and a MLSE receiver. The sampler samples the filtered electrical data signal twice each bit period with unequal sample spacings. For wide optical filtering bandwidths, the optimal sample spacing occurs at less than 50% of a bit period. For narrow bandwidths, the optimal sample instances occur closer to the maximum eye opening.