The present invention provides methods for producing recombinant peptides
in a bacterial host utilizing a mannitol, arabitol, glucitol, or
glycerol-inducible promoter, wherein the host bacterial cell that
produces the peptide has been rendered incapable of degrading or
metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues
thereof. The present invention provides bacterial cells that have been
genetically altered to inhibit the metabolism or degradation of mannitol,
glucitol, or arabitol, or derivatives or analogues thereof. The present
invention utilizes mannitol, arabitol, glucitol, or glycerol to induce
expression of a target polypeptide from an inducible promoter, allowing
for the use of an inexpensive and stable carbon source inducer in the
fermentation processes for the production of recombinant peptides.