The present invention provides a method of producing a transgenic hybrid
plant having no or reduced sexual reproduction, the method comprising:
(a) stably transforming a first plant with a first nucleic acid construct
comprising: (i) a promoter, P1; (ii) a site specific recombinase, RS1;
(iii) a promoter, P2; (iv) a selectable marker, SM1; (v) a promoter P3;
(vi) at least one nucleotide sequence the expression of which results in
no or reduced sexual reproduction, NRSR; and (vii) at least two target
sites, TRS2, specific for a site specific recombinase, RS2 that is
different from the site specific recombinase, RS1, and further wherein P1
is operably located upstream of RS1, P2 is operably located upstream of
SM1, P3 is operably located upstream, of NRSR, a first TRS2 is located
immediately downstream of P1 and a second TRS2 is located upstream of
NRSR; (b) stably transforming a second plant with a second nucleic acid
construct comprising: (i) a promoter, P4; (ii) a site specific
recombinase, RS2, that is different from the site specific recombinase,
RS1, of (a); (iii) a selectable marker, SM2; (iv) at least one nucleotide
sequence of interest, NOI; (v) at least two target sites, TRS1, specific
for the site specific recombinase, RS1; and (vi) at least two target
sites, TRS2, specific for the site specific recombinase, RS2, and further
wherein P4 is operably located upstream of SM2, RS2 is located downstream
of SM2, a first TRS1 is located immediately downstream of P4, a second
TRS1 is located immediately downstream of SM2, a first TRS2 is located
immediately upstream of RS2 and downstream of the second TRS1, a second
TRS2 is located immediately downstream of RS2 and the NOI is located
immediately downstream of the second TRS2; and (c) cross-pollinating the
stably transformed first plant of (a) with the stably transformed second
plant of (b) to produce a transgenic hybrid plant having no or reduced
sexual reproduction.