A system performs habituation analysis using central nervous system, autonomic nervous system, and effector data. Subjects are repeatedly exposed to stimulus material and data is collected using mechanisms such as Electroencephalography (EEG), Galvanic Skin Response (GSR), Electrocardiograms (EKG), Electrooculography (EOG), eye tracking, and facial emotion encoding. Data collected is analyzed to determine habituation and associated wear-out profiles for stimulus material.