A diaphragm pump for pumping a fluid includes a housing, a pump chamber defined in the housing, a first diaphragm for sealing the pump chamber, a pressure control chamber defined in the housing adjacent to the pump chamber via the first diaphragm, a second diaphragm for sealing the pressure control chamber, a drive mechanism operable to deform the first and second diaphragms, an introduction passage for introducing into the pressure control chamber a part of the fluid that is discharged from the pump chamber, and a pressure regulating valve disposed in the introduction passage for adjusting a pressure in the pressure control chamber to a medium pressure between a suction pressure and a discharge pressure.