Provided is a process, by which glucuronic acid and/or glucuronolactone can be produced in high yield, at low cost, in an easy manner and in safe. Also provided is a process for production of glucuronic acid and/or glucuronolactone which is characterized in that sucrose is oxidized to give sucrose carboxylic acid (and a salt thereof) (glucuronyl-fructoside, .beta.-D-fructosyl-(2.fwdarw.1)-.alpha.-D-glucuronic acid and a salt thereof), yeast is added so as to hydrolyze fructose residue of the sucrose carboxylic acid and to assimilate the resulting product, and thus produced glucuronic acid and/or glucuronolactone are/is collected.