The invention relates to a method of generating a signal indicative of the
presence of a target nucleic acid in a sample, where the method includes
forming a cleavage structure by incubating a sample comprising a target
nucleic acid with a probe having a secondary structure that changes upon
binding of the probe to the target nucleic acid and further comprising a
binding moiety. The invention also includes the steps of cleaving the
cleavage structure with a nuclease to release a nucleic acid fragment to
generate a signal, wherein generation of the signal is indicative of the
presence of a target nucleic acid in a sample, and detecting and/or
measuring the amount of the fragment captured by binding of a binding
moiety to a capture element on a solid support.The invention also relates
to a method of detecting or measuring a target nucleic acid in a sample,
where the method includes forming a cleavage structure by incubating a
sample containing a target nucleic acid with a probe having a secondary
structure that changes upon binding of the probe to a target nucleic acid
and comprising a binding moiety, and cleaving the cleavage structure with
a nuclease to generate a cleaved nucleic acid fragment and detecting
and/or measuring the amount of the fragment captured by binding of a
binding moiety to a capture element on a solid support.