A chromatographic separator characterized in that two pumps are employed for controlling chromatographic separation that combines a first process for supplying at least one of feed solution and eluent into a circulation system and extracting the same quantity as that of liquid supplied into the circulation system from one position of a packed column where some component is enriched in the circulation system, and a second process for circulating liquid in the system without supplying the feed solution and eluent and extracting the separated component at all. Easy-to-control chromatographic separator, in which a total number of pumps to be used is decreased and high separation performance is attained through a simple structure, can thereby be provided.

 
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> Method and apparatus for separating a target molecule from a liquid mixture

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