Systems and methods for creating data samples for data analysis. The method includes imaging at least a portion of a first object having a target portion to generate a first image, analyzing the first image by running a first series of algorithms using the first image to generate a first algorithm value cache, removing the target portion from the first object to form a modified first object, imaging at least a portion of the modified first object to generate a second image, analyzing the second image by running the first series of algorithms using the second image to generate a second algorithm value cache, generating a transformed result by running a subtraction process using the first algorithm value cache and the second algorithm value cache, and storing the transformed result in a knowledge base.