To provide a fuel cell which has a reaction layer having good reaction efficiency and an electron collection layer to efficiently collect electrons generated at the reaction layer and of which the output density is high and the characteristic is good and a method of manufacturing thereof, a fuel cell includes a first substrate provided with a gas flow path to supply a first reaction gas; a first electron collection layer formed on the first substrate; a first reaction layer formed on the first electron collection layer; an electrolyte film formed on the first reaction layer; a second reaction layer formed on the electrolyte film; a second electron collection layer formed on the second reaction layer; and a second substrate provided with a second gas flow path to supply a second reaction gas, the fuel cell including at least one of the first electron collection layer and the second electron collection layer being constructed by stacking conductive material particles. A method of manufacturing the fuel cell includes the first and/or the second electron collection layer formed by applying material to form the electron collection layer at predetermined intervals using a discharging device.