An A-to-T single nucleotide polymorphism (SNP) identified at position -134 relative to the ATG start codon, in the Pro-Melanin-Concentrating Hormone (PMCH) gene of Bos taurus and Bos indicus animals of both British and Continental type, is associated with changes in the average fat and grade fat. The A allele occurred in 67% of cattle examined and was associated with higher average fat and grade fat levels. The cattle industry may make use of these findings to genetically select for, and/or sort, cattle using this SNP.