The present invention provides new methods of delaying senescence in a
plant by inhibiting ACC oxidase, or EIN2 activity the plant. In
particular, it is directed to producing green leaves in maize through
inhibition of ethylene. The genes involved in producing this phenotype
include ACC deaminase, ethylene response sensor ("ERS"), ethylene
resistan ("ETR"), and ethylene insensitive ("EIN"). The delay in
senescence can be achieved through the production of ACC deaminase,
mutated ETR1 and ERS2 proteins, as well as overexpression of wild-type
ETR1 and ERS2 proteins.