A collapsible patient isolation pod for preventing flow of contaminants to or from a patient is disclosed. The isolation pod is preferably fabricated at least partially from a transparent, biochemically resistive material and includes a flexible enclosure that is configurable to receive a contaminated patient therein. The top is maintained spaced apart from the bottom such that the top is maintained out of physical contact with a contaminated patient received in the enclosure. The isolation pod has a plurality of wide, gloved access points to allow easy, multiple access points to a patient. A central connection manifold for oxygen, intravenous connections, or other fluids, may optionally be provided. Ventilation is provided within the isolation chamber with a negative pressure ventilation system and filtration on the air input near the patient's head, and provides filtration on output near the patient's feet. An integral pocket underneath the isolation pod may be provided to allow for a spine board or stretcher to be used. The invention provides a patient isolation system that permits health care providers safe and relatively unencumbered access to the isolated patient.