The present invention describes an alternative approach to increase GABA
production in prokaryotes or eukaryotes, namely by the insertion of the
putrescine catabolic pathway in organisms where the pathway does not
exist or has not clearly been identified. The invention describes methods
for the use of polynucleotides that encode functional putrescine
aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase
(GABAlde DeHase) polypeptides in plants to increase GABA production. The
preferred embodiment of the invention is in plants but other organisms
may be used. Changes in GABA availability will improve growth and
increase tolerance to biotic and abiotic stress.