A fruit guide assembly may include a frame to be positioned between a fruit conveyor and a multi-position fruit feeder, a vibrator removably fastened to the frame to be removable therefrom in the upward direction, and a fruit guide body removably fastened to the frame to be removable therefrom in an upward direction. The fruit guide body may have an upper surface with alternating ridges and valleys therein defining a plurality of fruit lanes. The fruit guide body may include spaced apart upper and bottom walls so that the fruit guide is a hollow fruit guide body in some embodiments. The hollow fruit guide body may comprise rotationally molded polyethylene, for example.