The present invention relates to methods of identifying, collecting and
isolating hematopoietic stem cells (HSCs) and compositions of purified
HSCs. Specifically, the present invention provides methods of isolating
and purifying CD150.sup.+ HSCs, CD48.sup.- HSCs, and CD244.sup.- HSCs.
The present invention also relates to purified cell samples with enriched
CD150.sup.+ HSCs, CD48.sup.- HSCs, and CD244.sup.- HSCs populations, as
well as methods of treating subjects with such compositions.