The invention provides an immunochromatographic test device for detecting a test substance in a sample, comprising a chromatographic membrane carrier; a sample developing member; and a labeling substance holding member; wherein a substance being capable of reacting to a human anti-mouse antibody is held in a member disposed at a position which is more upstream on the basis of the sample developing direction than the chromatographic membrane carrier, as well as an a method for detecting a test substance in a sample by using an immunochromatographic test device and a method for manufacturing an immunochromatographic test device.