Compositions and methods for nucleic acid amplification are provided that
minimize the formation and amplification of spurious products,
particularly in multiplex amplification reactions. Linear amplification
molecules are provided. A first embodiment comprises a first common
primer sequence, a first template homology region, a cleavable site, a
second common primer sequence and a second template homology region.
Methods employing the linear amplification molecules for nucleic acid
amplification are also provided. A first embodiment comprises a
template-specific hybridization, a linear amplification, a
template-specific intramolecular hybridization, a strand cleavage, a
second linear amplification and an exponential amplification using common
primers.