A metering device for aspirating and dispensing a liquid includes a
housing; a pumping medium, preferably a gas containing chamber contained
within the housing; a channel having a proximate end in fluid
communication with the chamber and a distal end in fluid communication
with an external environment; a heat or cold source providing a source of
heat or cold to the gas containing chamber; and a temperature sensor for
measuring the temperature inside the chamber. In a preferred embodiment,
the device further includes a pressure sensor for measuring gas pressure
inside the chamber, or multiple chambers. A method for aspirating and
dispensing a liquid includes: providing a metering device for aspirating
and dispensing a liquid that includes a housing; a gas containing chamber
contained within the housing, the chamber; a channel having a proximate
end in fluid communication with the chamber and a distal end in fluid
communication with an external environment; a heat or cold source
providing a source of heat or cold to the gas containing chamber; and a
temperature sensor for measuring the temperature inside the chamber;
providing a source of liquid to be aspirated; bringing the distal end of
the channel into contact with the liquid; cooling the gas containing
chamber with the heat or cold source to aspirate a first volume of liquid
into the device; and heating the gas containing chamber to dispense a
second volume of liquid out of the device. In a preferred embodiment, the
metering device is a used in a diagnostic analyzer for determining the
presence of one or more analytes in a sample.