The present invention relates to a peptide promoting cell adhesion and spreading, fragments, and derivatives thereof, more particularly, integrin-.alpha.3.beta.1-dependent peptide in human laminin-5 .alpha.3 chain LG3 domain, mediating integrin .alpha.3.beta.1 binding and cell adhesion and spreading, fragments and derivatives thereof. The inventive peptide, fragments and derivatives thereof can be used effectively for research on cell adhesion activity, wound care, tissue regeneration, inhibition of cancer metastesis etc. mediated by various extracellular matrix protein including laminin.