A tube holder (105) for use with a peristaltic pump includes a housing having a recess (165) for receipt of a pump rotor and a tube race around the recess. The tube race has a first race part and a second race part, a first tube inlet (167a) into the first race part and a first tube outlet (167b) from the first race part, a second tube inlet (167c) into the second race part and a second tube outlet (167d) from the second race part. The tube is insertable in the tube race by movement in a substantially orthogonal direction relative to the tube race so that it extends in through the first tube inlet (167a), around the first race part, out through the first tube outlet (167b), in through the second tube inlet (167c), around the second race part, and out through the second tube outlet (167d).