Devices and methods are provided for treating, preventing from growth, and neutralizing microorganisms using high-frequency, low-energy ultrasound. A particular method is provided for treating a fountain solution including simultaneously exposing the fountain solution to gas microbubbles and high-frequency ultrasound. A particular device is also provided, including a compartment for holding a reservoir of fountain solution, a gas microbubble emitter configured to emit microbubbles into the compartment, and a high-frequency ultrasound-emitter configured to emit ultrasound signals into the compartment. The high-frequency ultrasound may be higher than 100 kHz.