The invention relates to the field of regeneration of cells and the vegetative propagation of (micro)-organisms or specific parts such as tissues or organs thereof, for example of those cells grown in tissue or organ culture, and more in particular to the seedless propagation of plants. The invention provides a culture method for propagation of a plant from plant starting material wherein during regeneration of said starting material, especially in the phase of the development of the shoot-root body plan, root or shoot initiation is stimulated by a recombinant gene product or functional fragment thereof, for example derived from a gene involved in the regulation of plant development allowing reducing or omitting exogenous phytohormone addition to said culture.