This invention relates to the field of nucleic acid chemistry, more
specifically to the field of compositions and processes that are nucleic
acid analogs. More specifically, this invention relates to compositions
that allow the sequencing of oligonucleotides by synthesis, and processes
for sequencing by synthesis that exploit these compositions. Most
specifically, the instant invention discloses compositions of matter that
are 5'-triphosphates of ribo- and 2'-deoxyribonucleosides wherein the
3'-OH group is replaced by a 3'-ONHR group in the alpha configuration,
wherein R is either a H or CH.sub.3 group. Also disclosed are these
triphosphates where the nucleobase carries, via a linker, a reporter
groups, such as a fluorescent species that can be used in single- or
multi-copy DNA sequencing, or a tag that can be visualized by
ultramicroscopy. Also disclosed are processes that use these compositions
to do sequencing by synthesis.