The present invention provides methods of producing a clone non-human
mammalian nuclear transfer (NT) embryo and methods for producing a cloned
non-human mammal. Embodiments of the methods include introducing doner
genetic material into a metaphase I oocyte; introducing donor genetic
material into a non-enucleated oocyte; introducing donor genetic material
obtained from a donor cell that is a metaphase into an oocyte;
introducing donor genetic material into an oocyte, and naturally
activating the oocyte or the NT embryo; and introducing donor genetic
material obtained from a donor cell that is at late G1 phase into
anoocyte.