A method for the automated analysis of digital images, particularly for
the purpose of assessing the presence and severity of cancer in breast
tissue based on the relative proportions of tubule formations and
epithelial cells identified in digital images of histological slides. The
method includes the step of generating a property co-occurrence matrix
(PCM) from some or all of the pixels in the image, using the properties
of local mean and local standard deviation of intensity in neighbourhoods
of the selected pixels, and segmenting the image by labelling the
selected pixels as belonging to specified classes based upon analysis of
the PCM. In this way relatively dark and substantially textured regions
representing epithelial cells in the image can be distinguished from
lighter and more uniform background regions Other steps include
identifying groups of pixels representing duct cells in the image based
on intensity, shape and size criteria, dilating those pixels into
surrounding groups labelled as epithelial cells by a dimension to
correspond to an overall tubule formation, and calculating a metric based
on the ratio of the number of duct pixels after such dilation to the
total number of duct and epithelial pixels. Other uses for the method
could include the analysis of mineral samples containing certain types of
crystal formations.