The invention relates to a method for rapid detection of a target nucleic
acid amplification product while preventing cross-contamination between
target nucleic acid amplification products and avoiding false positives,
comprising the steps of: a) leaving the reaction tube unopened after the
amplification reaction is finished, so as to prevent the target nucleic
acid amplification product from leaking out and resulting in
contamination; b) placing the unopened reaction tube inside an enclosed
unit, making the target nucleic acid amplification product be transferred
to a test strip from the reaction tube in a physically enclosed
environment; c) performing detection in a visual read-out manner, and
determining the result; d) discarding the enclosed unit in a safety place
as a whole without opening it after the detection. The invention also
relates to a totally enclosed unit for detecting a target nucleic acid
amplification product, and still relates to applications of the totally
enclosed rapid detection unit in detection of infectious pathogens, food
industry, agriculture, livestock husbandry, customs quarantine control,
and determination of DNA.