Transformed cells are efficiently selected using a mutant ALS gene having
high specificity to PC herbicides. The transformation method comprises
the steps of: transforming a host cell with a recombination vector
containing a gene of interest and a gene coding for a mutant acetolactate
synthase having mutation of glycine corresponds to position 95 of the
amino acid sequence of a wild-type acetolactate synthase derived from
rice to alanine; culturing the transformed cell obtained in the former
step in the presence of a pyrimidinyl carboxy herbicide; and wherein the
gene coding for the mutant acetolactate synthase is used as a selection
marker.