The methods and compositions of the invention find use in the clinical
diagnosis of primary immunodeficiencies, particularly Wiskott-Aldrich
related syndromes. The compositions of the invention include isolated
nucleic acid molecules and oligonucleotide pairs suitable for use in
amplifying regions of the Wiskott-Aldrich syndrome protein gene and in
determining the nucleotide sequence of the Wiskott-Aldrich syndrome
protein gene in a patient. The invention facilitates efficient,
cost-effective amplification of one or more regions of the
Wiskott-Aldrich syndrome protein gene. The nucleotide sequence of
amplified DNA comprising one or more regions of the Wiskott-Aldrich
Syndrome Protein gene can be determined using the methods and
compositions of the invention. Knowledge of the patient's nucleotide
sequence in the Wiskott-Aldrich Syndrome Protein gene allows diagnosis of
the patient's primary immunodeficiency.