A method and composition made from extraction of the roots of Cissus Quadrangularis Linn plant is disclosed. The method comprises washing, drying and grinding the roots to provide a powdered root. A first solvent is added to the powdered root to extract a mixture comprising one or more keto-steroids and impurities. A second solvent is added to the mixture of the partially extracted powdered root and first solvent, wherein the one or more keto-steroids is substantially soluble in the second solvent and the impurities are soluble in the first solvent. A highly purified composition obtained by the method may be further combined with at least one excipient to provide a high yield keto-sterone composition having a predetermined keto-sterone content. A dose of the keto-sterone composition may be administered to a person to promote lean body mass, treat damaged or diseased connective tissue and provide an analgesic effect while reducing cortisol.