A bicycle frame includes a mandrel, a hub and freewheel assembly, two bearings, two first locking nuts, two fork end members, and two second locking nuts. Thus, when each of the first locking nuts is screwed onto the respective first threaded section of the mandrel and when each of the second locking nuts is screwed onto the respective second threaded section of the mandrel and rested on the respective fork end member, the respective fork end member will not be driven by the respective second locking nut to push the respective first locking nut to prevent the respective bearing from being compressed by the respective first locking nut so as to maintain the normal operation of the respective bearing.