A method for producing a polysaccharide and a conjugate vaccine including
the polysaccharide produced according to the method. A characteristic
step in the method is that the pH of the culture medium is kept at a
constant value with base or acid until adjustment with respectively base
or acid is not possible anymore. Using the method, capsular
polysaccharide may be obtained in a high yield in a relatively short
time. The method is straightforward, reproducible and cost-effective.