The invention relates to a device for measuring a Doppler frequency shift, including an optical signal channel delivering a signal light beam illuminating a reference medium; and a reference channel delivering a reference light beam for detecting the Doppler frequency shift. The frequency shift is determined from the difference in frequency between the light signal illuminating the reference medium and the light beam returned by the reference medium. The device includes at least two light radiation sources, preferably lasers, with one light radiating source generating the light beam for the signal channel and the other source generating the light beam for the reference channel.