The present invention provides methods to confer resistance to
protoporphyrinogen-inhibiting herbicides onto crop plants. Resistance is
conferred by genetically engineering the plants to express cloned DNA
encoding a protoporphyrinogen oxidase resistant to porphyric herbicides.
If such resistant crop plants are cultivated, utilization of these
herbicides on fields of these crop plants becomes feasible. This should
allow for simpler and more effective weed management, and increase the
value of these herbicides for agricultural use. Furthermore, the present
invention provides plants, algae, plant cells, and algal cells which have
been made resistant to protoporphyrinogen oxidase-inhibiting herbicides
by the subject methods using a herbicide-resistant protoporphyrinogen
oxidase gene that has been prepared by genetic engineering methods. In
addition, the present invention provides methods to evaluate the
inhibitory effects of test compounds on protoporphyrinogen oxidase
activity, as well as methods to identify protoporphyrinogen oxidase
inhibitors among test compounds. Preferred cloned DNA fragments encoding
protoporphyrinogen oxidase enzymes resistant to porphyric herbicides are
also described.