A method for performing an immunoassay is described. The method is
particularly useful for detecting extracellular polysaccharide (EPS)
and/or lipopolysaccharide (LPS) producing microorganisms. The method is
particularly useful for detecting microorganisms which produce
extracellular polysaccharides (EPS) also known as exocellular
polysaccharides, capsule, and/or lipopolysaccharides (LPS). In a
preferred method for detecting microorganisms which produce EPS, LPS, or
both, the EPS and/or LPS is extracted from a sample with
cetyltrimethylammonium bromide (CTAB) to produce molecular aggregates
which are then preferentially bound to colored polystyrene latex
particles over other components in the sample, and the bound EPS and/or
LPS detected using a lateral flow immunoassay apparatus which has
immobilized thereon antibodies specific for the EPS and/or LPS. The
method can also be used to detect particular viruses, for example viruses
of the potyviridae or tobamoviridae group.