Described herein are novel polynucleotides associated with liver cancer.
The polynucleotides are miRNAs, miRNA precursors, and associated nucleic
acids. Methods and compositions are described that can be used for
diagnosis, prognosis, and treatment of liver cancer. Also described
herein are methods that can be used to identify modulators of the
disease-associated polynucleotides. Also described herein are methods and
compositions for linear amplification and labeling of a targeted nucleic
acid. The amplified targeted molecules may be used in hybridization
techniques like Luminex and Microarray analysis.