RNA interference is provided for inhibition of ocular hypertension target mRNA expression for lowering elevated intraocular pressure in patients with open-angle glaucoma or ocular hypertension. Ocular hypertension targets include carbonic anhydrase II, IV, and XII; .beta.1- and .beta.2 adrenergic receptors; acetylcholinesterase; Na.sup.+/K.sup.+-ATPase; and Na--K-2Cl cotransporter. Ocular hypertension is treated by administering interfering RNAs of the present invention.