A light diffusion module and a back light module using the same. The light diffusion module is disposed corresponding to the light source module of the back light module. The light diffusion module includes a first diffusion layer and the second diffusion layer. The first diffusion layer is disposed on top of the light source module and the top light exit surface has a plurality of first micro structures juxtapositioned to each other. The second diffusion layer is disposed on top of the first diffusion layer, and the top surface has a plurality of second micro structures juxtapositioned to each other. The ratio of the width of each first micro structure to the width of each second micro structure is between 1.1 and 1.8. The ratio of the height of each first micro structure to the height of each second micro structure is between 0.8 and 1.5.